FCGR1 Protein (Biotin,His-Avi Tag)

Catalog No. ABIN7320414

Recombinant FCGR1 protein expressed in HEK-293 Cells.

Quick Overview for FCGR1 Protein (Biotin,His-Avi Tag) (ABIN7320414)

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This FCGR1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Recombinant Mouse CD64/FCGR1 Protein (His&AVI Tag), Biotinylated(Active)

Sequence: Met 1-Pro 297

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind mouse APCS in a functional ELISA.2. Labeling ratio of biotin to protein: 1.1

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FCGR1 (Fc Receptor, IgG, High Affinity I (FCGR1))

Alternative Name: CD64/FCGR1

Background:

Background: High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Synonym: AI323638;AV092959;CD64;FcgammaRI;IGGHAFC

Molecular Weight: 34.3 kDa

NCBI Accession: NP_034316

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process