Fibronectin Protein (AA 1270-1546, AA 1721-2016)

Catalog No. ABIN7318495

This Recombinant Fibronectin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Fibronectin Protein (AA 1270-1546, AA 1721-2016) (ABIN7318495)

Target: Fibronectin

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1270-1546, AA 1721-2016

Purpose: Recombinant Human Fibronectin/FN Protein

Sequence: Pro1270-Ser1546&Ala1721-Thr2016

Characteristics: Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED₅₀ for this effect is 0.1-0.5 μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 12.5 mM Citric acid, 1.25 % Sucrose, 0.1 % Tween 80, pH 5.5 .
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Fibronectin

Alternative Name: Fibronectin/FN

Background: CIG,ED-B,FINC,FN,FN1,FNZ,Fibronectin,GFND,GFND2,LETS,MSF,Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.

Molecular Weight:

Calculated MW: 62.7 kDa

Observed MW: 60-80 kDa

Gene ID: 2335

UniProt: P02751