GLB1 Protein (AA 1-1024)

Catalog No. ABIN7320988

This Recombinant GLB1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for GLB1 Protein (AA 1-1024) (ABIN7320988)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-1024

Purpose: Recombinant E.coli Beta-galactosidase Protein

Sequence: Met1-Lys1024(12-41AA deletion)

Characteristics: Recombinant E.coli Beta-galactosidase(M443L, C500S) is produced by our E.coli expression system and the target gene encoding Met1-Lys1024(12-41AA deletion) is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: E.coli Beta-galactosidase

Background: Beta-gal,Beta-galactosidase,Lactase,lacZ,β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. β-galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose.

Molecular Weight:

Calculated MW: 112.9 kDa

Observed MW: 115 kDa

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process