Grancalcin Protein
Quick Overview for Grancalcin Protein (ABIN7318548)
Target
See all Grancalcin (GCA) ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human Grancalcin/GCA Protein
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Sequence
- Met 1-Ile217
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Characteristics
- Recombinant Human Grancalcin is produced by our E.coli expression system and the target gene encoding Met1-Ile217 is expressed.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) protein (His tag)
custom-made GCA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-220) protein (His tag)custom-made GCA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) protein (His tag)GCA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) protein (His-IF2DI Tag)GCA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) protein (GST tag)GCA Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))
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Alternative Name
- Grancalcin/GCA
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Background
- GCA,GCL,Grancalcin,Grancalcin (GCA) is a cytoplasmic granule membrane protein that contains 4 EF-hand domains. GCA is calcium-binding protein and particularly abundant in human neutrophils. GCA is highly expressed in bone marrow, and it can be detected in neutrophils and macrophages. Calcium-binding protein GCA cooperates with SRI and LCP1, so it may play a role in the adhesion of neutrophils to fibronectin. GCA also may play a role in the formation of focal adhesions.
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Molecular Weight
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Calculated MW: 24.0 kDa
Observed MW: 26 kDa
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Gene ID
- 25801
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UniProt
- P28676
Target
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