K-RAS Protein (His tag)

Catalog No. ABIN7319807

This Recombinant K-RAS protein is produced in Escherichia coli (E. coli).

Quick Overview for K-RAS Protein (His tag) (ABIN7319807)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This K-RAS protein is labelled with His tag.

Purpose: Recombinant Human KRAS(G12C, N-6His)

Sequence: Thr2-Cys185(Gly12Cys)

Characteristics: Recombinant Human GTPase Kras is produced by our E.coli expression system and the target gene encoding Thr2-Cys185(Gly12Cys) is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Target Type: Viral Protein

Background:

Background: K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.

Synonym: Ki-Ras, c-K-ras, KRAS2, RASK2, CFC2

Molecular Weight: 23.6 kDa