HIST1H3A Protein

Catalog No. ABIN7317606

Recombinant HIST1H3A protein expressed in Escherichia coli (E. coli).

Quick Overview for HIST1H3A Protein (ABIN7317606)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human Histone H3.1/HIST1H3A/H3FA Protein

Sequence: Met 1-Ala 136

Characteristics: A DNA sequence encoding the native human HIST1H3A (NP_003520.1) (Met1-Ala136) was expressed. Human and Mouse HIST1H3A sequences are identical.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 2 mM β-Mercaptoethanol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: Histone H3.1/HIST1H3A/H3FA

Background: H3/A,H3FA,HIST1H3A,HIST1H3B,HIST1H3C,HIST1H3D,HIST1H3E,HIST1H3F,HIST1H3G,HIST1H3H,HIST1H3I,HIST1H3J,Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of thehistone H3 family which is a core component of nucleosome. It is expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Molecular Weight:

Calculated MW: 15.5 kDa

Observed MW: 15.5 kDa

Gene ID: 8350

NCBI Accession: NP_003520

UniProt: P68431