IL1A Protein (AA 115-270)

Catalog No. ABIN7321259

Recombinant IL1A protein expressed in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (AA 115-270) (ABIN7321259)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 115-270

Purpose: Recombinant Rat IL-1A/ IL-1α Protein

Sequence: Ser115-Ser270

Characteristics: Recombinant Rat Interleukin-1 Alpha is produced by our E.coli expression system and the target gene encoding Ser115-Ser270 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1A/ IL-1alpha

Background: Interleukin,Hematopoietin,IL1F1hematopoietin,IL1F,IL-1F,alpha,BAF,Hematopoietin-1,IL1,IL1 alpha,IL-1 alpha,IL1-A,IL-1A,IL1-ALPHA,IL1F1,IL-1F1,IL1F1hematopoietin-1,interleukin 1,interleukin-1 alpha,LAF,LEM,preinterleukin 1 alpha,pro-interleukin-1-alpha,Il1a,FAF,Hematopoietin 1,IL 1 alpha,IL 1A,IL1,IL1 ALPHA,IL1F1,Interleukin 1 alpha,LAF,LEM,Preinterleukin 1 alpha,Il1a,Interleukin 1 (IL-1) is a name that designates two proteins, IL-1αand IL-1β, which are the products of distinct genes, but which show approximately 25 % amino acid (aa) sequence identity and which recognize the same cell surface receptors. IL-1αand IL-1β are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1α remains in the cytosol of cells, although there is evidence for a membranebound form of the precursor form of IL-1α. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporally upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen.

Molecular Weight:

Calculated MW: 17.9 kDa

Observed MW: 16 kDa

Gene ID: 24493

UniProt: P16598

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints