IL17F Protein (His tag)

Catalog No. ABIN7321075

This Recombinant IL17F protein is produced in Baculovirus infected Insect Cells.

Quick Overview for IL17F Protein (His tag) (ABIN7321075)

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This IL17F protein is labelled with His tag.

Purpose: Recombinant Rat Interleukin-17F/IL-17F Protein (His Tag)(Active)

Sequence: Met1-Ala153

Characteristics: A DNA sequence encoding the rat Il17f (NP_001015011.1) (Met1-Ala153) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized rat IL17F-His at 10 μg/mL (100 μL/well) can bind Rat IL17RA-Fc3 . The EC50 of Rat IL17RA-Fc3 is 0.28-0.66 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: Interleukin-17F/IL-17F

Background:

Background: Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells, and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also function in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Synonym: IL17F,IL-17F

Molecular Weight: 16.4 kDa

NCBI Accession: NP_001015011

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity