IL23 Protein (AA 22-196, AA 23-335)

Catalog No. ABIN7320867

This Recombinant IL23 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for IL23 Protein (AA 22-196, AA 23-335) (ABIN7320867)

Target: IL23 (Interleukin 23 (IL23))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 22-196, AA 23-335

Purpose: Recombinant Mouse IL-23

Sequence: Val22-Ala196&Met23-Ser335

Characteristics: Recombinant Mouse Interleukin-23 is produced by our Baculovirus expression system and the target geneencoding Val22-Ala196&Met23-Ser335 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED₅₀ for this effect is 125.44 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL23 (Interleukin 23 (IL23))

Alternative Name: IL-23

Background: CLMF p40,IL-12 subunit p40,IL-23p19,NKSF2,SGRF,Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.

Molecular Weight:

Calculated MW: 19.7&35.8 kDa

Observed MW: 40&20 kDa

Gene ID: 83430

UniProt: Q9EQ14, P43432