KEAP1 Protein (GST tag,His tag,AVI tag)

Catalog No. ABIN7317444

Product Details

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This KEAP1 protein is labelled with GST tag,His tag,AVI tag.

Purpose: Recombinant Human KEAP1/INRF2 Protein (His & GST & AVI Tag)

Sequence: Gln 2-Cys 624

Characteristics: A DNA sequence encoding the human KEAP1 (Q14145) (Gln 2-Cys 624) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus linked by the AVI tag.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1/INRF2 (KEAP1 Products)

Synonyms: INrf2 Protein, KLHL19 Protein, INRF2 Protein, mKIAA0132 Protein, Inrf2 Protein, fb36f11 Protein, keap1 Protein, wu:fb36f11 Protein, KEAP1 Protein, klhl19 Protein, zgc:171654 Protein, kelch like ECH associated protein 1 Protein, kelch-like ECH-associated protein 1 Protein, Kelch-like ECH-associated protein 1 Protein, kelch-like ECH-associated protein 1a Protein, kelch like ECH associated protein 1 L homeolog Protein, kelch-like ECH-associated protein 1b Protein, KEAP1 Protein, Keap1 Protein, keap1a Protein, keap1 Protein, keap1.L Protein, keap1b Protein

Background:

Background: Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.

Synonym: INRF2,KEAP-1,KLHL19

Molecular Weight: 99.2 kDa

UniProt: Q14145

Pathways: Maintenance of Protein Location