KEAP1 Protein (GST tag,His tag,AVI tag)

Catalog No. ABIN7317444

This Recombinant KEAP1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for KEAP1 Protein (GST tag,His tag,AVI tag) (ABIN7317444)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This KEAP1 protein is labelled with GST tag,His tag,AVI tag.

Purpose: Recombinant Human KEAP1/INRF2 Protein (His & GST & AVI Tag)

Sequence: Gln 2-Cys 624

Characteristics: A DNA sequence encoding the human KEAP1 (Q14145) (Gln 2-Cys 624) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus linked by the AVI tag.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1/INRF2

Background:

Background: Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.

Synonym: INRF2,KEAP-1,KLHL19

Molecular Weight: 99.2 kDa

UniProt: Q14145

Pathways: Maintenance of Protein Location