KEAP1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all KEAP1 Proteins
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KEAP1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human KEAP1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product KEAP1 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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Static and turnover kinetic measurement of protein biomarkers involved in triglyceride metabolism including apoB48 and apoA5 by LC/MS/MS." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1179-87, (2016) (PubMed).
: "Comparative Tissue Proteomics of Microdissected Specimens Reveals Novel Candidate Biomarkers of Bladder Cancer." in: Molecular & cellular proteomics : MCP, (2015) (PubMed).
: "Chitinase 3-like 1: prognostic biomarker in clinically isolated syndromes. ..." in: Brain : a journal of neurology, Vol. 138, Issue Pt 4, pp. 918-31, (2015) (PubMed).
: "Stk38 protein kinase preferentially inhibits TLR9-activated inflammatory responses by promoting MEKK2 ubiquitination in macrophages." in: Nature communications, Vol. 6, pp. 7167, (2015) (PubMed).
: "A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1." in: Retrovirology, Vol. 11, pp. 121, (2015) (PubMed).
: "Amniotic Fluid Soluble Myeloid Differentiation-2 (sMD-2) as Regulator of Intra-amniotic Inflammation in Infection-induced Preterm Birth." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 73, Issue 6, pp. 507-21, (2015) (PubMed).
: "Ginger compound [6]-shogaol and its cysteine-conjugated metabolite (M2) activate Nrf2 in colon epithelial cells in vitro and in vivo." in: Chemical research in toxicology, Vol. 27, Issue 9, pp. 1575-85, (2014) (PubMed).
: "TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein." in: Nature, Vol. 516, Issue 7529, pp. 116-20, (2014) (PubMed).
: "Chemoproteomic profiling of lysine acetyltransferases highlights an expanded landscape of catalytic acetylation." in: Journal of the American Chemical Society, Vol. 136, Issue 24, pp. 8669-76, (2014) (PubMed).
: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).
: "CaMKII mediates recruitment and activation of the deubiquitinase CYLD at the postsynaptic density." in: PLoS ONE, Vol. 9, Issue 3, pp. e91312, (2014) (PubMed).
: "LTBP-2 competes with tropoelastin for binding to fibulin-5 and heparin, and is a negative modulator of elastinogenesis." in: Matrix biology : journal of the International Society for Matrix Biology, Vol. 34, pp. 114-23, (2014) (PubMed).
: "Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells." in: Cytotherapy, Vol. 16, Issue 8, pp. 1132-44, (2014) (PubMed).
: "HNRNPA1 interacts with a 5'-flanking distal element of interleukin-6 and upregulates its basal transcription." in: Genes and immunity, Vol. 14, Issue 8, pp. 479-86, (2013) (PubMed).
: "Screening citrullinated proteins in synovial tissues of rheumatoid arthritis using 2-dimensional western blotting." in: The Journal of rheumatology, Vol. 40, Issue 3, pp. 219-27, (2013) (PubMed).
: "Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 48, pp. 18880-92, (2013) (PubMed).
: "Identification of UV-protective activators of nuclear factor erythroid-derived 2-related factor 2 (Nrf2) by combining a chemical library screen with computer-based virtual screening." in: The Journal of biological chemistry, Vol. 287, Issue 39, pp. 33001-13, (2012) (PubMed).
: "Cisplatin-induced ototoxicity is mediated by nitroxidative modification of cochlear proteins characterized by nitration of Lmo4." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18674-86, (2012) (PubMed).
: "An ATP-site on-off switch that restricts phosphatase accessibility of Akt." in: Science signaling, Vol. 5, Issue 223, pp. ra37, (2012) (PubMed).
: "LPLUNC1 modulates innate immune responses to Vibrio cholerae." in: The Journal of infectious diseases, Vol. 204, Issue 9, pp. 1349-57, (2011) (PubMed).
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Static and turnover kinetic measurement of protein biomarkers involved in triglyceride metabolism including apoB48 and apoA5 by LC/MS/MS." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1179-87, (2016) (PubMed).
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- Target
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
- Alternative Name
- Keap1 (KEAP1 Products)
- Synonyms
- INrf2 Protein, KLHL19 Protein, INRF2 Protein, mKIAA0132 Protein, Inrf2 Protein, fb36f11 Protein, keap1 Protein, wu:fb36f11 Protein, KEAP1 Protein, klhl19 Protein, zgc:171654 Protein, kelch like ECH associated protein 1 Protein, kelch-like ECH-associated protein 1 Protein, Kelch-like ECH-associated protein 1 Protein, kelch-like ECH-associated protein 1a Protein, kelch like ECH associated protein 1 L homeolog Protein, kelch-like ECH-associated protein 1b Protein, KEAP1 Protein, Keap1 Protein, keap1a Protein, keap1 Protein, keap1.L Protein, keap1b Protein
- Background
- This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
- Molecular Weight
- 69.5 kDa
- NCBI Accession
- NP_987096
- Pathways
- Maintenance of Protein Location
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