KIT Ligand Protein (KITLG) (His tag)

Catalog No. ABIN7321261

Recombinant KIT Ligand protein expressed in Escherichia coli (E. coli).

Quick Overview for KIT Ligand Protein (KITLG) (His tag) (ABIN7321261)

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This KIT Ligand protein is labelled with His tag.

Purpose: Recombinant Rat SCF/c-Kit Ligand Protein (E.coli, His Tag)

Sequence: Gln26-Ala189

Characteristics: Recombinant Rat Stem Cell Factor is produced by our E.coli expression system and the target gene encoding Gln26-Ala189 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: <1.0 EU per μg as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: SCF/c-Kit Ligand

Background:

Background: Stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.

Synonym: FPH2, KIT ligand, Kitl, KITLG, KL-1, Mast cell growth factor, MGF, MGFSHEP7, SCF, SFc-Kit ligand, SLF, steel factor, Stem Cell Factor, c-kit Ligand

Molecular Weight: 19.3 kDa

UniProt: P21581

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway