Latexin Protein (LXN) (His tag)

Catalog No. ABIN7196736

This Recombinant Latexin protein is produced in Escherichia coli (E. coli).

Quick Overview for Latexin Protein (LXN) (His tag) (ABIN7196736)

Target: Latexin (LXN)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Latexin protein is labelled with His tag.

Purpose: Recombinant Human Latexin/LXN Protein (His Tag)

Sequence: Glu 2-Glu222

Characteristics: A DNA sequence encoding the human Latexin (NP_064554.3) (Glu 2-Glu222) was expressed, fused with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM HEPES, 0.1M KCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Latexin (LXN)

Alternative Name: Latexin/LXN

Background: ECI,TCI,Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to theprotease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.

Molecular Weight:

Calculated MW: 26.8 kDa

Observed MW: 26.8 kDa

Gene ID: 56925

NCBI Accession: NP_064554

UniProt: Q9BS40