LCAT Protein (AA 25-440) (His tag)

Catalog No. ABIN7318716

This Recombinant LCAT protein is expressed in HEK-293 Cells.

Quick Overview for LCAT Protein (AA 25-440) (His tag) (ABIN7318716)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 25-440

Purification tag / Conjugate: This LCAT protein is labelled with His tag.

Purpose: Recombinant Human LCAT Protein (His Tag)

Sequence: Phe25-Glu440

Characteristics: Recombinant Human Lecithin-cholesterol acyltransferase is produced by our Mammalian expression system and the target gene encoding Phe25-Glu440 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: LCAT

Background: Phosphatidylcholine-sterol acyltransferase,Phospholipid-cholesterol acyltransferase and LACT,also named Lecithin-cholesterol acyltransferase,is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.,Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes,followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).

Molecular Weight:

Calculated MW: 48.1 kDa

Observed MW: 66 kDa

Gene ID: 3931

UniProt: P04180

Pathways: Lipid Metabolism