LYPLA2 Protein (His tag)
Quick Overview for LYPLA2 Protein (His tag) (ABIN7319283)
Target
See all LYPLA2 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This LYPLA2 protein is labelled with His tag.
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Purpose
- Recombinant Human LYPLA2 Protein (His Tag)
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Sequence
- Met 1-Val231
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Characteristics
- Recombinant Human Acyl-Protein Thioesterase 2 is produced by our E.coli expression system and the target gene encoding Met1-Val231 is expressed with a 6His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Lysophospholipase II (LYPLA2) (AA 1-231) protein (His tag)
custom-made LYPLA2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophospholipase II (LYPLA2) (AA 1-231) protein (His tag)custom-made LYPLA2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophospholipase II (LYPLA2) (AA 1-231) protein (GST tag)LYPLA2 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Lysophospholipase II (LYPLA2) protein (Myc-DYKDDDDK Tag)LYPLA2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 100 mM Nacl, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- LYPLA2 (Lysophospholipase II (LYPLA2))
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Alternative Name
- LYPLA2
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Background
- APT-2,APT2,Acyl-Protein Thioesterase 2,LPL-II,LYPLA2,LysoPLA II,Lysophospholipase II,Acyl-Protein Thioesterase 2 is a cytoplasmic protein that belongs to the AB Hydrolase 2 family. LYPLA2 is a lysophospholipase and hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. LYPLA2 has hydrolase activity that converts Palmitoyl-protein to palmitate and protein. LYPLA2 regulates the multifunctional lysophospholipids by acting on biological membranes. LYPLA2 participates in Glycerophospholipid metabolism pathway, fatty acid metabolic process and lipid metabolic process.
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Molecular Weight
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Calculated MW: 25.8 kDa
Observed MW: 28 kDa
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Gene ID
- 11313
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UniProt
- O95372
Target
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