MMP3 Protein

Catalog No. ABIN7196949

Product Details

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human MMP-3 Protein (Active)

Sequence: Tyr 18-Thr 272

Characteristics: A DNA sequence encoding the human MMP3 (AAA36321.1) N-terminal fragment (Tyr 18-Thr 272) was expressed and purified.

Purity: > 97 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K(Dnp)-NH2, AnaSpec, Catalog # 27114.The specific activity is >300 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 10 mM CaCL2, 1uM ZnCL2, 50 mM NaCl, 0.5 % Brij35, pH 7.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP-3 (MMP3 Products)

Synonyms: CHDS6 Protein, MMP-3 Protein, SL-1 Protein, STMY Protein, STMY1 Protein, STR1 Protein, SLN-1 Protein, SLN1 Protein, STR-1 Protein, Stmy1 Protein, Str1 Protein, MMP10 Protein, chds6 Protein, mmp-3 Protein, mmp13 Protein, mmp3 Protein, sl-1 Protein, stmy Protein, stmy1 Protein, str1 Protein, matrix metallopeptidase 3 Protein, matrix metallopeptidase 3 (stromelysin 1, progelatinase) Protein, matrix metallopeptidase 3 L homeolog Protein, MMP3 Protein, Mmp3 Protein, mmp3.L Protein

Background:

Background: Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP3 can also active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.

Synonym: Stromelysin-1, SL-1, Matrix metalloproteinase-3,Transin-1, MMP3, STMY1,CHDS6,MMP-3,SL-1,STMY,STR1

Molecular Weight: 29 kDa