MAP4K2 Protein (His-GST)

Catalog No. ABIN7317061

Recombinant MAP4K2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for MAP4K2 Protein (His-GST) (ABIN7317061)

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 87 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This MAP4K2 protein is labelled with His-GST.

Purpose: Recombinant Human MAP4K2/GC Kinase Protein (His & GST Tag)

Sequence: Met 1-Tyr 812

Characteristics: A DNA sequence encoding the human MAP4K2 (AAH47865.1) (Met 1-Tyr 812) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Alternative Name: MAP4K2/GC Kinase

Background: BL44,GCK,MAP4K2,RAB8IP,Mitogen-activated protein kinase kinase kinase kinase 2, also known as B lymphocyte serine/threonine-protein kinase, Germinal center kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, Rab8-interacting protein and MAP4K2, is a cytoplasm and peripheral membrane protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K2 contains oneCNH domain and oneprotein kinase domain. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. MAP4K2 can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. It is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1 / MEKK1. MAP4K2 enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. It may also play a role in the regulation of vesicle targeting or fusion.

Molecular Weight:

Calculated MW: 119 kDa

Observed MW: 116 kDa

Gene ID: 5871

UniProt: Q12851