MAP4K5 Protein (His-GST)

Catalog No. ABIN7317063

This Recombinant MAP4K5 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for MAP4K5 Protein (His-GST) (ABIN7317063)

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 82 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This MAP4K5 protein is labelled with His-GST.

Purpose: Recombinant Human MAP4K5/MEKKK5 Protein (His & GST Tag)

Sequence: Met 1-Tyr 846

Characteristics: A DNA sequence encoding the human MAP4K5 (NP_006566.2) (Met 1-Tyr 846) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM TCEP, pH 8.5

Preservative: PMSF

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Alternative Name: MAP4K5/MEKKK5

Background: GCKR,KHS,KHS1,MAPKKKK5,Mitogen-activated protein kinase kinase kinase kinase 5, also known as Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5 and MAP4K5, is a cytoplasm protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K5 is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate. It contains oneCNH domain and oneprotein kinase domain. MAP4K5 is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. MAP4K5 has been shown to interact with CRKL and TRAF2. This kinase was shown to activate Jun kinase in mammalian cells. MAP4K5 is an early component of MAP kinase signal cascades. It may play a role in the response to environmental stress. MAP4K5 appears to act upstream of the JUN N-terminal pathway.

Molecular Weight:

Calculated MW: 123 kDa

Observed MW: 116 kDa

Gene ID: 11183

NCBI Accession: NP_006566

UniProt: Q9Y4K4