NGFR Protein (Fc Tag)

Catalog No. ABIN7320090

This Recombinant NGFR protein is produced in HEK-293 Cells.

Quick Overview for NGFR Protein (Fc Tag) (ABIN7320090)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This NGFR protein is labelled with Fc Tag.

Purpose: Recombinant Mouse NGFR/CD271 Protein (Fc Tag)(Active)

Sequence: Met 1-Asn 243

Characteristics: A DNA sequence encoding the extracellular domain of mouse NGFR (Q9Z0W1) (Met 1-Asn 243) was fused with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 2 ng/mL Recombinant mouse NGF.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR/CD271

Background:

Background: Nerve growth factor receptors (NGFRs) belong to a large growth factor receptor family. NGFR includes two types of receptors: high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. High-affinity nerve growth factor receptor is also referred as Trk familywhose members are bound by some neurotrophins with high affinity. Nerve growth factor binds with TrkA after being released from target cells, the NGF / TrkA complex is subsequently trafficked back to the cell body. The Low-affinity nerve growth factor receptor also named p75 which binds with all kinds of neurotrophins with low affinity. All the four kinds of neurotrophins, including Nerve growth factor, Brain derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75. Studies have proved that NGFR acts as a molecular signal swith that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

Synonym: LNGFR,p75,p75NGFR,p75NTR,RP23-67E18.6,Tnfrsf16

Molecular Weight: 50.6 kDa

UniProt: Q9Z0W1

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding