NCF2 Protein (His-GST)
Quick Overview for NCF2 Protein (His-GST) (ABIN7317175)
Target
See all NCF2 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This NCF2 protein is labelled with His-GST.
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Purpose
- Recombinant Human NCF2/P67phox Protein (His & GST Tag)
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Sequence
- Met 1-Val526
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Characteristics
- A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Neutrophil Cytosolic Factor 2 (NCF2) (AA 1-526) protein (GST tag)
NCF2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neutrophil Cytosolic Factor 2 (NCF2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NCF2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Neutrophil Cytosolic Factor 2 (NCF2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NCF2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Neutrophil Cytosolic Factor 2 (NCF2) proteinNCF2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))
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Alternative Name
- NCF2/P67phox
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Background
- NCF-2,NOXA2,P67-PHOX,P67PHOX,NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.
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Molecular Weight
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Calculated MW: 87.6 kDa
Observed MW: 80-90 kDa
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Gene ID
- 4688
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UniProt
- P19878
Target
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