MGMT Protein (His tag)

Catalog No. ABIN7318122

This Recombinant MGMT protein is expressed in Escherichia coli (E. coli).

Quick Overview for MGMT Protein (His tag) (ABIN7318122)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This MGMT protein is labelled with His tag.

Purpose: Recombinant Human MGMT Protein (His Tag)

Sequence: Met 1-Asn207

Characteristics: Recombinant Human Methylated-DNA--protein-cysteine methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mMTrisHCl,1 mM EDTA, pH 8.0 .

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background:

Background: MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Synonym: Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase,O-6-methylguanine-DNA-alkyltransferase, MGMT

Molecular Weight: 23.8 kDa

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus