OBFC1 Protein (His tag)

Catalog No. ABIN7318845

This Recombinant OBFC1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for OBFC1 Protein (His tag) (ABIN7318845)

Target: OBFC1 (Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This OBFC1 protein is labelled with His tag.

Purpose: Recombinant Human OBFC1/STN1 Protein (His Tag)

Sequence: Met 1-Phe368

Characteristics: Recombinant Human Oligosaccharide-Binding Fold-Containing Protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Phe368 is expressed with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 5 % Trehalose, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: OBFC1 (Oligonucleotide/oligosaccharide-Binding Fold Containing 1 (OBFC1))

Alternative Name: OBFC1/STN1

Background: CST Complex Subunit STN1,OBFC1,Oligonucleotide/Oligosaccharide-Binding Fold-Containing Protein 1,STN1,Suppressor of Cdc Thirteen Homolog,CST Complex Subunit STN1 (OBFC1) is a 368 amino acid protein that contains one OB DNA-binding domain. It is a member of the STN1 family. OBFC1 is component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites.

Molecular Weight:

Calculated MW: 44.3 kDa

Observed MW: 44 kDa

Gene ID: 79991

UniProt: Q9H668