PAK3 Protein (PAK3) (His tag)
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- Target See all PAK3 Proteins
- PAK3
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PAK3 protein is labelled with His tag.
- Purpose
- Recombinant Human PAK3 Protein (His Tag)(Active)
- Sequence
- Met 1-Arg 544
- Characteristics
- A DNA sequence encoding the human PAK3 isoform 2 (O75914-2) (Met 1-Arg 544) was fused with a polyhistidine tag at the C-terminus.
- Purity
- > 80 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 98 nmol/min/mg using MBP as substrate.
- Top Product
- Discover our top product PAK3 Protein
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PAK3 (PAK3) (AA 1-544) (Active) protein (GST tag)
PAK3 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
PAK3 (PAK3) (AA 1-544) (Active) protein (His-GST)PAK3 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
PAK3 (PAK3) (AA 1-90) protein (GST tag)PAK3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
PAK3 (PAK3) (AA 1-559) protein (Strep Tag)Crystallography grade PAK3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
PAK3 (PAK3) (AA 1-559) protein (Strep Tag)Crystallography grade PAK3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- PAK3
- Alternative Name
- PAK3 (PAK3 Products)
- Synonyms
- CDKN1A Protein, MRX30 Protein, MRX47 Protein, OPHN3 Protein, PAK3beta Protein, bPAK Protein, hPAK3 Protein, PAK-3 Protein, Pak65alpha Protein, Pak65beta Protein, Stk4 Protein, beta-PAK Protein, XPak3 Protein, bpak Protein, cdkn1a Protein, mrx30 Protein, mrx47 Protein, ophn3 Protein, pak3 Protein, pak3-A Protein, pak3beta Protein, p21 (RAC1) activated kinase 3 Protein, p21 protein (Cdc42/Rac)-activated kinase 3 Protein, p21 protein (Cdc42/Rac)-activated kinase 3 L homeolog Protein, PAK3 Protein, Pak3 Protein, pak3.L Protein
- Background
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Background: PAK3 is a member of PAK proteins, a family of serine/threonine p21-activating kinases, serve as effectors of small Rho GTPases Cdc42 and RAC and have been implicated in a wide range of biological activities. There are six mammalian PAKs which can be divided into two groups: group I PAKs (PAK1-3) and group II PAKs (PAK4-6). Although the two PAK groups are architecturally similar there are differences in their mode of regulation suggesting their cellular functions are likely to be different. Group I p21-activated kinases (PAK1/2/3) is demonstrated as ERK3/ERK4 activation loop kinases. It has been shown that group I PAKs phosphorylate ERK3 and ERK4 on Ser-189 and Ser-186, respectively, both in vitro and in vivo, and that expression of activated Rac1 augments this response. Besides regulation enzymatic activation of ERK3/ERK4, PAKs can also play roles in downstream activation of MAP kinase-activated protein kinase 5 (MK5) in vivo. Thus, the group I PAKs act as upstream activators of ERK3 and ERK4 and unravel a novel PAK-ERK3/ERK4-MK5 signaling pathway. In clinical, PAK has been proposed as a potential therapeutic target in schwannomas.
Synonym: bPAK;CDKN1A;hPAK3;MRX30;MRX47;OPHN3;PAK3beta
- Molecular Weight
- 62 kDa
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