PDE1B Protein (His-GST)

Catalog No. ABIN7317495

This Recombinant PDE1B protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PDE1B Protein (His-GST) (ABIN7317495)

Target: PDE1B (phosphodiesterase 1B, Calmodulin-Dependent (PDE1B))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PDE1B protein is labelled with His-GST.

Purpose: Recombinant Human PDE1B Protein (His & GST Tag)

Sequence: Met 1-Asp 536

Characteristics: A DNA sequence encoding the human PDE1B long isoform (Q01064-1) (Met 1-Asp 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PDE1B (phosphodiesterase 1B, Calmodulin-Dependent (PDE1B))

Alternative Name: PDE1B

Background: PDE1B1,PDES1B,Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, also known as Cam-PDE 1B and PDE1B, is a cytoplasm protein which belongs to thecyclic nucleotide phosphodiesterase family and PDE1 subfamily. Phosphodiesterase-10A (PDE10A), Phosphodiesterase-1B (PDE1B), Phosphodiesterase-4B (PDE4B), and Phosphodiesterase-4A (PDE4A) are important regulators of signal transduction in striatum due to their catalysis of cyclic AMP and cyclic GMP. PDE1B is highly expressed in the striatum. It binds two divalent metal cations per subunit. Site one of PDE1B may preferentially bind zinc ions, while site two of PDE1B has a preference for magnesium and/or manganese ions. PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. It has a preference for cGMP as a substrate.

Molecular Weight:

Calculated MW: 89.2 kDa

Observed MW: 75 kDa

Gene ID: 5153

UniProt: Q01064

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma