PDGF-AA Homodimer (Active) protein (His tag)

Catalog No. ABIN7318911

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for PDGF-AA Homodimer (Active) protein (His tag) (ABIN7318911)

Target: PDGF-AA Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human PDGF-AA Protein (His Tag)(Active)

Sequence: Ser87-Thr 211

Characteristics: Recombinant Human Platelet-derived growth factor AA is produced by our E.coli expression system and the target gene encoding Ser87-Thr211 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using BALB/C-3T3 mouse fibroblast cells.  The ED50 for this effect is 100-400 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDGF-AA Homodimer

Alternative Name: PDGF-AA

Background:

Background: Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing, Signaling is modulated by the formation of heterodimers with PDGFB.

Synonym: Platelet-derived growth factor subunit A,PDGF subunit A,PDGF-1,Platelet-derived growth factor A chain,Platelet-derived growth factor alpha polypeptide, PDGFA,PDGF1

Molecular Weight: 15.9 kDa

UniProt: P04085