PVRL2 Protein (His tag)

Catalog No. ABIN7320318

Recombinant PVRL2 protein expressed in HEK-293 Cells.

Quick Overview for PVRL2 Protein (His tag) (ABIN7320318)

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 98 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This PVRL2 protein is labelled with His tag.

Purpose: Recombinant Mouse CD112/Nectin-2 Protein (His Tag)(Active)

Sequence: Met 1-Gly 351

Characteristics: A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.1. Immobilized mouse CD112-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse DNAM1-His with a linear range of 0.156-5.0 μg/ml.2. Immobilized mouse CD112-His at 10 μg/ml (100 μl/well) can bind mouse DNAM1-Fc with a linear range of 0.03-1.0 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Alternative Name: CD112/Nectin-2

Background:

Background: Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Synonym: AI325026 Protein, AI987993 Protein, Cd112 Protein, MPH Protein, nectin-2 Protein, Pvr Protein, Pvs Protein

Molecular Weight: 36 kDa

NCBI Accession: NP_033016

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization