PVRL2 Protein (AA 32-360) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN5526673

Product Details

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 32-360

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PVRL2 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: PrecisionAvi

Sequence: AA 32-360

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 64.0 kDa. As a result of glycosylation, the protein migrates as 66 KDa under reducing (R) condition, and 130 KDa under non-reducing (NR) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Alternative Name: Nectin-2 (PVRL2 Products)

Synonyms: prr2 Protein, AI325026 Protein, AI987993 Protein, Cd112 Protein, MPH Protein, Pvr Protein, Pvs Protein, nectin-2 Protein, CD112 Protein, HVEB Protein, PRR2 Protein, PVRR2 Protein, nectin cell adhesion molecule 2 Protein, Nectin2 Protein, NECTIN2 Protein

Background: Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Molecular Weight: 64.0 kDa

NCBI Accession: NP_002847

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization