PVRL4 Protein (Fc Tag)

Catalog No. ABIN7320868

This Recombinant PVRL4 protein is produced in Human Cells.

Quick Overview for PVRL4 Protein (Fc Tag) (ABIN7320868)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PVRL4 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse Nectin-4 (C-Fc)

Sequence: Gly31-Ser349

Characteristics: Recombinant Mouse Poliovirus Receptor-Related Protein  4 is produced by our Mammalian expression system and the target gene encoding Gly31-Ser349 is expressed with a Fc tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Immobilized Recombinant Mouse Nectin-4 (C-6His)(Cat#PKSM041376) at 10μg/ml (100 μl/well) can bind Recombinant Mouse Nectin-4 (C-Fc)(Cat#PKSM041375). The ED50 of PKSM041375 is 0.75 ug/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: Nectin-4

Background:

Background: Nectin-4(PVRL4)isatypeItransmembraneglycoproteinwhichbelongstothenectinfamilyofIgsuperfamilyproteins.ItcontainstwoIg-likeC2-typedomainsandoneIg-likeV-typedomain.PVRL4seemstobeinvolvedincelladhesionthroughtrans-homophilicand-heterophilicinteractions,thelatterincludingspecificallyinteractionswithnectin-1.Itdoesnotactasreceptorforalpha-herpesvirusentryintocells.Itispredominantlyexpressedinplacenta,theembryoandbreastcarcinoma.Butitisnotdetectedinnormalbreastepithelium.Thesolubleformisproducedbyproteolyticcleavageatthecellsurface(shedding),probablybyADAM17.Mutationsinthisgenearethecauseofectodermaldysplasia-syndactylysyndrometype 1, an autosomal recessive disorder.

Synonym: PVRL4, Nectin-4, Ig superfamily receptor LNIR, Poliovirus receptor-related protein 4, PRR4, LNIR

Molecular Weight: 61.4kDa

UniProt: Q8R007

Pathways: Cell-Cell Junction Organization