PVRL4 Protein (AA 32-351) (His-Avi Tag,Biotin)
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- Target See all PVRL4 Proteins
- PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-351
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PVRL4 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human Nectin-4 Protein
- Sequence
- Gly32-Val351
- Characteristics
- Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))
- Alternative Name
- Nectin-4 (PVRL4 Products)
- Synonyms
- PVRL4 Protein, si:ch211-155e24.1 Protein, 1200017F15Rik Protein, Prr4 Protein, RGD1559826 Protein, EDSS1 Protein, LNIR Protein, PRR4 Protein, nectin-4 Protein, nectin cell adhesion molecule 4 Protein, NECTIN4 Protein, nectin4 Protein, Nectin4 Protein
- Background
- Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
- Molecular Weight
- 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
- UniProt
- Q96NY8
- Pathways
- Cell-Cell Junction Organization
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