PFN2 Protein
Quick Overview for PFN2 Protein (ABIN7318935)
Target
See all PFN2 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human Profilin-2/PFN2 Protein
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Sequence
- Met 1-Phe140
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Characteristics
- Recombinant Human Profilin-2 is produced by our E.coli expression system and the target gene encoding Met1-Phe140 is expressed.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Profilin 2 (PFN2) (AA 1-140) protein (GST tag)
PFN2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Profilin 2 (PFN2) (AA 1-140) protein (His tag)PFN2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Profilin 2 (PFN2) (AA 1-140) protein (His-IF2DI Tag)PFN2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Profilin 2 (PFN2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PFN2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- PFN2 (Profilin 2 (PFN2))
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Alternative Name
- Profilin-2/PFN2
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Background
- PFL,PFN2,Profilin-2,Profilin-II,Profilin-II (PFN2) is ubiquitous protein which belongs to the profilin family. PFN2 binds to actin, thenaffects the structure of the cytoskeleton. At high concentrations, profiling prevents the polymerization of actin, while increases that at low concentrations. PFN2 is a ubiquitous actin monomer-binding protein. It regulates actin polymerization in response to extra cellular signals. PFN2 binds to PIP2, it inhibits the formation of IP3 and DG.
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Molecular Weight
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Calculated MW: 15.0 kDa
Observed MW: 14 kDa
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Gene ID
- 5217
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UniProt
- P35080
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Pathways
- Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
Target
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