PRM2 Protein (His tag)

Catalog No. ABIN7318947

This Recombinant PRM2 protein is expressed in HEK-293 Cells.

Quick Overview for PRM2 Protein (His tag) (ABIN7318947)

Target: PRM2 (Protamine 2 (PRM2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PRM2 protein is labelled with His tag.

Purpose: Recombinant Human Protamine-2/PRM2 Protein (His Tag)

Sequence: Met 1-Phe389

Characteristics: Recombinant Human Ribonucleotide Reductase Small Chain is produced by our Mammalian expression system and the target gene encoding Met1-Phe389 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 5 % Trehalose, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PRM2 (Protamine 2 (PRM2))

Alternative Name: Protamine-2/PRM2

Background: RR2,RRM2,Ribonucleoside-Diphosphate Reductase Subunit M2,Ribonucleotide Reductase Small Chain,Ribonucleotide Reductase Small Subunit,Ribonucleoside-Diphosphate Reductase Subunit M2 (RRM2) belongs to the ribonucleoside diphosphate reductase small chain family. The reductase of RRM2 catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. RRM2 supplies the precursors essential for DNA synthesis. RRM2 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.

Molecular Weight:

Calculated MW: 45.9 kDa

Observed MW: 47 kDa

Gene ID: 6241

UniProt: P31350