POMGNT1 Protein (AA 59-660) (His tag)

Catalog No. ABIN7318922

This Recombinant POMGNT1 protein is produced in HEK-293 Cells.

Quick Overview for POMGNT1 Protein (AA 59-660) (His tag) (ABIN7318922)

Target: POMGNT1 (Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 59-660

Purification tag / Conjugate: This POMGNT1 protein is labelled with His tag.

Purpose: Recombinant Human POMGNT1 Protein (His Tag)

Sequence: Leu59-Thr660

Characteristics: Recombinant Human Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1 is produced by our Mammalian expression system and the target gene encoding Leu59-Thr660 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10 % Glycerol, pH 8.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: POMGNT1 (Protein O-Linked Mannose Beta1,2-N-Acetylglucosaminyltransferase (POMGNT1))

Alternative Name: POMGNT1

Background: 2-N-Acetylglucosaminyltransferase 1,2-N-Acetylglucosaminyltransferase I.2,MGAT1.2,POMGNT1,POMGnT1,Protein O-Linked-Mannose Beta-1,UDP-GlcNAc:Alpha-D-Mannoside Beta-1,Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).

Molecular Weight:

Calculated MW: 69.3 kDa

Observed MW: 74 kDa

Gene ID: 55624

UniProt: Q8WZA1