RHEB Protein (His tag)
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- Target See all RHEB Proteins
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This RHEB protein is labelled with His tag.
- Purpose
- Recombinant Human RHEB Protein (His Tag)
- Sequence
- Met 1-Cys181
- Characteristics
- A DNA sequence encoding the human RHEB (Q15382)(Met1-Cys181) was fused with a polyhistide tag at the N-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (His tag)
RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Ras Homolog Enriched in Brain (RHEB) (AA 1-181) protein (T7 tag)RHEB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Ras Homolog Enriched in Brain (RHEB) (AA 1-184) protein (GST tag)RHEB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ras Homolog Enriched in Brain (RHEB) protein (Myc-DYKDDDDK Tag)RHEB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- RHEB (Ras Homolog Enriched in Brain (RHEB))
- Alternative Name
- RHEB (RHEB Products)
- Background
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Background: RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
Synonym: GTP-Binding Protein Rheb, Ras Homolog Enriched in Brain, RHEB, RHEB2
- Molecular Weight
- 22.4 kDa
- UniProt
- Q15382
- Pathways
- RTK Signaling
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