RNASET2 Protein (His tag)

Catalog No. ABIN7197719

Recombinant RNASET2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for RNASET2 Protein (His tag) (ABIN7197719)

Target: RNASET2 (Ribonuclease T2 (RNASET2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This RNASET2 protein is labelled with His tag.

Purpose: Recombinant Human RNASET2 Protein (Baculovirus, His Tag)

Sequence: Met 1-His 256

Characteristics: A DNA sequence encoding the human RNASET2 isoform 1 (O00584-1) (Met 1-His 256) was fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RNASET2 (Ribonuclease T2 (RNASET2))

Alternative Name: RNASET2

Background: 3.1.27.-,RNASE6PL,Ribonuclease 6,Ribonuclease T2,RNASET2 (ribonuclease T2) is an enzyme which belongs to the RNase T2 family. It is highly expressed in the temporal lobe and fetal brain. RNASET2 gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27, a region associated with human malignancies and chromosomal rearrangement. Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly. An infantile-onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications.

Molecular Weight:

Calculated MW: 28.5 kDa

Observed MW: 35 kDa

Gene ID: 8635

UniProt: O00584