S100A1 Protein

Catalog No. ABIN7197741

This Recombinant S100A1 protein is produced in Escherichia coli (E. coli).

Quick Overview for S100A1 Protein (ABIN7197741)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human S100A1 Protein

Sequence: Met 1-Ser 94

Characteristics: A DNA sequence encoding the human S100A1 (NP_006262.1) (Met 1-Ser 94) was expressed.

Sterility: 0.2 μm filtered

Biological Activity Comment: Immobilized recombinant human Fc-S100B at 10 μg/mL (100 μl/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL. 3. Measured by its ability to bind human His-S100B in functional ELISA.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A1

Background: S100,S100-alpha,S100A,S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation.This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation.. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

Molecular Weight: 10.5 kDa

Gene ID: 6271

NCBI Accession: NP_006262

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins