S100A9 Protein
Quick Overview for S100A9 Protein (ABIN7319331)
Target
See all S100A9 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purpose
- Recombinant Human S100A9 Protein
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Sequence
- Thr 2-Pro114
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Characteristics
- Recombinant Human S100 Calcium Binding Protein A9 is produced by our E.coli expression system and the target gene encoding Thr2-Pro114 is expressed.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized Recombinant Human S100A9(PKSH033380) at 2μg/ml (100 μl/well) can bind Anti-Human S100A9 Antibody. The ED50 of Anti-Human S100A9 Antibody is 0.58μg/ml.
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S100 Calcium Binding Protein A9 (S100A9) (AA 2-114) (Active) protein (His tag)
S100A9 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) protein (GST tag)S100A9 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
S100 Calcium Binding Protein A9 (S100A9) (AA 99-330) protein (Fc Tag)S100A9 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) (Active) protein (His tag)S100A9 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE Active
S100 Calcium Binding Protein A9 (S100A9) (AA 1-114) protein (His-IF2DI Tag)S100A9 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
S100 Calcium Binding Protein A9 (S100A9) (AA 1-113) protein (His-IF2DI Tag)S100A9 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
S100 Calcium Binding Protein A9 (S100A9) protein (Myc-DYKDDDDK Tag)S100A9 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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Alternative Name
- S100A9
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Background
- CAGB,CFAG,Calgranulin-B,Calprotectin L1H subunit,Leukocyte L1 complex heavy chain,MRP-14,Protein S100-A9,Protein S100-A9 (also MRP14 and calgranulin B)is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response.It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism.
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Molecular Weight
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Calculated MW: 13.2 kDa
Observed MW: 14 kDa
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NCBI Accession
- NP_002956
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Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
Target
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