SHPK Protein (His tag)
Quick Overview for SHPK Protein (His tag) (ABIN7318998)
Target
See all SHPK ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This SHPK protein is labelled with His tag.
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Purpose
- Recombinant Human Sedoheptulokinase/SHPK Protein (His Tag)
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Sequence
- Met 1-Ser478
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Characteristics
- Recombinant Human Sedoheptulokinase is produced by our Mammalian expression system and the target gene encoding Met1-Ser478 is expressed with a 6His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Sedoheptulokinase (SHPK) (AA 1-478) protein (His tag)
custom-made SHPK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Sedoheptulokinase (SHPK) (AA 1-476) protein (His tag)custom-made SHPK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Sedoheptulokinase (SHPK) (AA 1-478) protein (GST tag)SHPK Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 10 % Glycerol, 3 mM DTT, pH 7.4.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- SHPK (Sedoheptulokinase (SHPK))
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Alternative Name
- Sedoheptulokinase/SHPK
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Background
- CARKL,Carbohydrate kinase-like protein,SHK,SHPK,Sedoheptulokinase,Sedoheptulokinase (SHPK) belongs to the FGGY kinase family, and is mainly located in cytoplasm. SHPK is strongly expressed in liver, kidney and pancreas. It is expressed at lower levels in placenta and heart, and very weakly expressed in lung and brain. SHPK catalyzes the chemical reaction: ATP + sedoheptulose = ADP + sedoheptulose 7-phosphatecan, It can transform sedoheptulose to sedoheptulose 7-phosphate in the condition of ATP, and acts as a modulator of macrophage activation through control of glucose metabolism. In addition, It also can be down-regulated by LPS.
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Molecular Weight
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Calculated MW: 52.5 kDa
Observed MW: 53 kDa
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Gene ID
- 23729
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UniProt
- Q9UHJ6
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Pathways
- Cellular Response to Molecule of Bacterial Origin
Target
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