STK40 Protein (His-GST)

Catalog No. ABIN7197936

This Recombinant STK40 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for STK40 Protein (His-GST) (ABIN7197936)

Target: STK40 (serine/threonine Kinase 40 (STK40))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This STK40 protein is labelled with His-GST.

Purpose: Recombinant Human STK40 Protein (His & GST Tag)

Sequence: Met 1-Lys 435

Characteristics: A DNA sequence encoding the human STK40 (NP_114406) (Met1-Lys435) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, 3 mM DTT, 0.5M Urea, 0.5 mM GSH, pH 8.0

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: STK40 (serine/threonine Kinase 40 (STK40))

Alternative Name: STK40

Background: SHIK;SgK495,STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NFκB-mediated gene transcription. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.

Molecular Weight:

Calculated MW: 76.8 kDa

Observed MW: 85 kDa

Gene ID: 83931

NCBI Accession: NP_114406

UniProt: Q8N2I9