SIGLEC10 Protein (AA 19-543) (Fc Tag)

Catalog No. ABIN7320907

This Recombinant SIGLEC10 protein is expressed in HEK-293 Cells.

Quick Overview for SIGLEC10 Protein (AA 19-543) (Fc Tag) (ABIN7320907)

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-543

Purification tag / Conjugate: This SIGLEC10 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse Siglec-10 (C-Fc)

Sequence: Met19-Lys543

Characteristics: Recombinant Mouse Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met19-Lys543 is expressed with a Fc tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Alternative Name: Siglec-10

Background: MGC126774,PRO940,SIGLEC10,SLG2,SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7,Siglec-10,Siglec-G,Siglec-like protein 2,Siglec10,SiglecG,sialic acid-binding Ig-like lectin 10,siglec-like gene 2,Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Molecular Weight:

Calculated MW: 85.6 kDa

Observed MW: 110-135 kDa

UniProt: Q80ZE3