SRPK1 Protein (His-GST)

Catalog No. ABIN7317393

Recombinant SRPK1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for SRPK1 Protein (His-GST) (ABIN7317393)

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This SRPK1 protein is labelled with His-GST.

Purpose: Recombinant Human SRPK1 Protein (His & GST Tag)

Sequence: Glu 2-Ser 655

Characteristics: A DNA sequence encoding the human SRPK1 (AAH38292.1) (Glu 2-Ser 655) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % Glycerol, 0.1 mMEDTA, 0.5 mM PMSF, 1 mM TCEP, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Alternative Name: SRPK1

Background: RP3-422H11.1,SFRSK1,Serine / threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein which belongs to theprotein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

Molecular Weight:

Calculated MW: 102 kDa

Observed MW: 120 kDa

Gene ID: 6732

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades