SUV420H2/KMT5C Protein (His-GST)

Catalog No. ABIN7317449

Recombinant SUV420H2/KMT5C protein expressed in Escherichia coli (E. coli).

Quick Overview for SUV420H2/KMT5C Protein (His-GST) (ABIN7317449)

Target: SUV420H2/KMT5C (SUV420H2) (Suppressor of Variegation 4-20 Homolog 2 (SUV420H2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This SUV420H2/KMT5C protein is labelled with His-GST.

Purpose: Recombinant Human SUV420H2 Protein (His & GST Tag)

Sequence: Gly 2-Leu 280

Characteristics: A DNA sequence encoding the amino acid sequence (Gly 2-Leu 280) of human SUV420H2 (NP_116090.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 0.5M NaCl, 30 % Glycerol, 0.05 % Teween, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SUV420H2/KMT5C (SUV420H2) (Suppressor of Variegation 4-20 Homolog 2 (SUV420H2))

Alternative Name: SUV420H2

Background: KMT5C,Histone-lysine N-methyltransferase SUV420H2, also known as Suppressor of variegation 4-20 homolog 2, Su(var)4-20 homolog 2, Lysine N-methyltransferase 5C, SUV420H2 and KMT5C, is nucleus protein which belongs to thehistone-lysine methyltransferase family and Suvar4-20 subfamily. SUV420H2 is a histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. SUV420H2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). FRAP experiments reveal that SUV420H2 is strongly associated to pericentric heterochromatin. The fraction of SUV420H2 captured and characterized by TAP/MS is a soluble fraction which may be in a stable association with HP1. SUV420H2 may be recruited to heterochromatin in association with HP1, and stably maintained at its heterochromatin sites in an HP1-independent fashion.

Molecular Weight:

Calculated MW: 60.0 kDa

Observed MW: 60 kDa

Gene ID: 84787

NCBI Accession: NP_116090

UniProt: Q86Y97