Syndecan 1 Protein (SDC1) (His tag)

Catalog No. ABIN7320206

This Recombinant Syndecan 1 protein is produced in HEK-293 Cells.

Quick Overview for Syndecan 1 Protein (SDC1) (His tag) (ABIN7320206)

Target: Syndecan 1 (SDC1)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Syndecan 1 protein is labelled with His tag.

Purpose: Recombinant Mouse Syndecan-1/SDC1 Protein (His Tag)

Sequence: Met 1-Glu 252

Characteristics: A DNA sequence encoding the extracellular domain of mouse SDC1 (NP_035649.1) (Met 1-Glu 252) was expressed, with a C-terminal polyhistidine tag.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Syndecan 1 (SDC1)

Alternative Name: Syndecan-1/SDC1

Background: AA408134,AA409076,CD138,Sstn,Synd,Synd1,syn-1,Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.

Molecular Weight:

Calculated MW: 26 kDa

Observed MW: 45-60 kDa

Gene ID: 20969

NCBI Accession: NP_035649

UniProt: P18828

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism