Thrombopoietin Protein (THPO) (His tag)

Catalog No. ABIN7319081

Recombinant Thrombopoietin protein expressed in Human Cells.

Quick Overview for Thrombopoietin Protein (THPO) (His tag) (ABIN7319081)

Target: Thrombopoietin (THPO)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Thrombopoietin protein is labelled with His tag.

Purpose: Recombinant Human Thrombopoietin/TPO Protein (His Tag)(Active)

Sequence: Ser22-Gly353

Characteristics: Recombinant Human Thrombopoietin is produced by our Mammalian expression system and the target gene encoding Ser22-Gly353 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using MO7E human megakaryocytic leukemic cells. The ED50 for this effect is 0.55 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl, pH 8.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin/TPO

Background:

Background: Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Synonym: Thrombopoietin,C-mpl ligand,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,Myeloproliferative leukemia virus oncogene ligand,THPO

Molecular Weight: 37.3 kDa

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity