TIMP1 Protein

Catalog No. ABIN7198249

Product Details

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purpose: Recombinant Rat TIMP-1/TIMP1 Protein (Active)

Sequence: Met1-Ala217

Characteristics: A DNA sequence encoding the rat TIMP1 (P01860) (Met1-Ala217) was expressed and purified.

Purity: > 97 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2, AnaSpec Catalog # 27076.The IC50 value is approximately 4 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP-1/TIMP1 (TIMP1 Products)

Synonyms: TIMP-1 Protein, TIMP1 Protein, DKFZp468A0912 Protein, CLGI Protein, EPA Protein, EPO Protein, HCI Protein, TIMP Protein, Timp Protein, Clgi Protein, TIMP metallopeptidase inhibitor 1 Protein, tissue inhibitor of metalloproteinase 1 Protein, TIMP1 Protein, Timp1 Protein

Background:

Background: TIMP metallopeptidase inhibitor 1, also known as TIMP-1/TIMP1, Collagenase inhibitor 16C8 fibroblast Erythroid-potentiating activity, TPA-S1TPA-induced proteinTissue inhibitor of metalloproteinases 1, is a natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. TIMP-1/TIMP1 is found in fetal and adult tissues. Highest levels are found in bone, lung, ovary and uterus. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 mediates erythropoiesis in vitro, but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this protein encoding gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This encoding gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-1/TIMP1 is Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.

Synonym: TIMP1,Timp-1

Molecular Weight: 21.5 kDa

UniProt: P01860