TLK1 Protein (His-GST)

Catalog No. ABIN7317054

This Recombinant TLK1 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for TLK1 Protein (His-GST) (ABIN7317054)

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 88 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This TLK1 protein is labelled with His-GST.

Purpose: Recombinant Human TLK1/PKU-beta Protein (His & GST Tag)

Sequence: Met 1-Tyr 766

Characteristics: A DNA sequence encoding the human TLK1 isoform 1 (Q9UKI8-1) (Met 1-Tyr 766) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 1. 6 nmol/min/mg using Histone H3 protein as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Alternative Name: TLK1/PKU-beta

Background: KIAA0137,PKU-beta,The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Calculated MW: 114 kDa

Observed MW: 125 kDa

Gene ID: 9874

UniProt: Q9UKI8