TCN2 Protein (His tag)

Catalog No. ABIN7198283

Recombinant TCN2 protein expressed in HEK-293 Cells.

Quick Overview for TCN2 Protein (His tag) (ABIN7198283)

Target: TCN2 (Transcobalamin II (TCN2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This TCN2 protein is labelled with His tag.

Purpose: Recombinant Mouse TCN2 Protein (His Tag)

Sequence: Met 1-Trp 430

Characteristics: A DNA sequence encoding the mouse TCN2 (O88968) (Met 1-Trp 430) was expressed, with a C-terminal polyhistidine tag.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: TCN2 (Transcobalamin II (TCN2))

Alternative Name: TCN2

Background: AW208754,Tcn-2,Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl, vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

Molecular Weight:

Calculated MW: 47.0 kDa

Observed MW: 42 kDa

UniProt: O88968