TWF1 Protein

Catalog No. ABIN7317476

Recombinant TWF1 protein expressed in Escherichia coli (E. coli).

Quick Overview for TWF1 Protein (ABIN7317476)

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human TWF1/Twinfilin-1 Protein

Sequence: Met 1-Asp 252

Characteristics: A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Alternative Name: TWF1/Twinfilin-1

Background: A6,MGC23788,MGC41876,PTK9,Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein which belongs to theactin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65 % sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Molecular Weight:

Calculated MW: 29 kDa

Observed MW: 36 kDa

Gene ID: 5756

UniProt: Q12792

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location