UBE2A Protein (His-GST)
Quick Overview for UBE2A Protein (His-GST) (ABIN7319273)
Target
See all UBE2A (ube2a) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This UBE2A protein is labelled with His-GST.
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Purpose
- Recombinant Human UBE2A Protein (GST & His Tag)
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Sequence
- Met 1-Cys152
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Characteristics
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 A is produced by our E.coli expression system and the target gene encoding Met1-Cys152 is expressed with a GST, 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Ubiquitin-Conjugating Enzyme E2A (ube2a) (AA 1-152) protein (His tag)
custom-made ube2a Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2A (ube2a) (AA 1-152) protein (GST tag)ube2a Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Conjugating Enzyme E2A (ube2a) (AA 1-152) protein (His tag)ube2a Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Ubiquitin-Conjugating Enzyme E2A (ube2a) (AA 1-152) protein (His-IF2DI Tag)ube2a Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))
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Alternative Name
- UBE2A
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Background
- HR6A,RAD6 Homolog A,RAD6A,UBE2A,Ubiquitin Carrier Protein A,Ubiquitin-Conjugating Enzyme E2 A,Ubiquitin-Protein Ligase A,hHR6A,Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC.
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Molecular Weight
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Calculated MW: 49.0 kDa
Observed MW: 45 kDa
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Gene ID
- 7319
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UniProt
- P49459
Target
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