UBE2H Protein

Catalog No. ABIN7198586

Recombinant UBE2H protein expressed in Escherichia coli (E. coli).

Quick Overview for UBE2H Protein (ABIN7198586)

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 93 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human Ube2H Protein

Sequence: Met 1-Leu 183

Characteristics: A DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Alternative Name: Ube2H

Background: E2-20K,GID3,UBC8,UBCH,UBCH2,UBE2H,UbcH2,Ubiquitin Carrier Protein H,Ubiquitin-Conjugating Enzyme E2 H,Ubiquitin-Conjugating Enzyme E2-20K,Ubiquitin-Protein Ligase H,UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.

Molecular Weight:

Calculated MW: 21 kDa

Observed MW: 21 kDa

Gene ID: 7328

UniProt: P62256