YES1 Protein (His-GST)

Catalog No. ABIN7317062

Recombinant YES1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for YES1 Protein (His-GST) (ABIN7317062)

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This YES1 protein is labelled with His-GST.

Purpose: Recombinant Human c-Yes/YES1 Protein (His & GST Tag)

Sequence: Gly 2-Leu 543

Characteristics: A DNA sequence encoding the human YES1 (NP_005424.1) (Gly 2-Leu 543) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 35 nmol/min/mg using Poly(Glu, Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, 0.5 mM TCEP, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Alternative Name: c-Yes/YES1

Background: HsT441,P61-YES,Yes,c-yes,Proto-oncogene tyrosine-protein kinase Yes, also known as Proto-oncogene c-Yes, p61-Yes and YES1, is a cytoplasm protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and SRC subfamily. YES1 / c-Yes contains oneprotein kinase domain, oneSH2 domain and oneSH3 domain. It is thought that the subcellular distribution of Src-family tyrosine kinases, including c-Yes binding to the cellular membrane, is membranous and/or cytoplasmic. YES1 / c-Yes protein tyrosine kinase is known to be related to malignant transformation. YES1 / c-Yes and c-Src are the two most closely related members of the Src family of nonreceptor tyrosine kinases. Although there is much evidence to support redundancy in signaling between these two kinases. YES1 / c-Yes promotes formation of the tight junction by phosphorylating occludin, while c-Src signaling downregulates occludin formation in a Raf-1 dependent manner. YES1 / c-Yes has tyrosine kinase activity. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP / CD46.

Molecular Weight:

Calculated MW: 88.5 kDa

Observed MW: 75 kDa

Gene ID: 7525

NCBI Accession: NP_005424

UniProt: P07947

Pathways: CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling